Applied biosystems identifiler plus user manual

Fluorescently labelled amplicons were spectrally resolved by capillary. Applied biosystems address, contact number of applied. Comparison of identifiler, identifiler plus and minifiler. View online or download applied biosystems 7500 fast installation and maintenance manual, getting started. Manuals and user guides for applied biosystems 7500. Optimized for casework, the ampflstr identifiler plus pcr amplification kit enables forensic analysts to.

Life technologiesapplied biosystems globalfiler 6dye liz600 size standard fga a vwa d18s51 d19s433 d8s1179 d21s11 d3s58 th01 d16s539. Applied biosystems steponeplus manuals manuals and user guides for applied biosystems steponeplus. The allele count per sample increases significantly due to the number of ministrs and robustness of the master mix included in the globalfiler kit. Email promega technical services if you have questions on use of this system. Validation experiments to evaluate the performance of this ce consisted of a precision study, sensitivity study, genotype concordance, a two person mixture study, and signal normalization.

We have 1 applied biosystems steponeplus manual available for free pdf download. Allele frequencies for the 15 str loci included in the ampflstr identifiler pcr amplification kit panel from applied biosystems d3s58, vwa, fga, d8s1179, d21s11, d18s51, d5s818, ds317, d7s820, th01, tpox, csf1po, d19s433, d2s38, d16s539 and several statistical parameters were estimated from a sample of 425 unrelated individuals representing all ethnic groups in morocco berbers. We have 4 applied biosystems 7500 manuals available for free pdf download. As an example, the following proto col was used for sample preparation and electrophoresis on the. Identifiler applied biosystems and identifiler plus applied. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. Applied biosystems provides preformulated, readytouse, qualitytested, taqman assays for use with the 7500 system. Applied biosystems ampf str identifiler plus pcr amplification kit and the globalfiler kit casework. Bowflex ultimate users manual download bowflex ultimate users manual. The pcr amplification reaction mixture was prepared in a volume of 25 ll containing 10 llofampfstr identifiler plus master mix, 5 llof ampfstr identifiler plus primer set, and a. Includes the user guide manual for the ampflstr identifiler plus pcr. Installation and maintenance manual, getting started, getting started manual. Identifiler and identifiler plus thermal cycling protocols followed manufacturer recommendations found in each kits manual, and was carried out on the geneamp 9700 thermal cycler applied biosystems.

The applied biosystems globalfiler and globalfiler iqc pcr amplification kits are 6dye, short tandem repeat str multiplex assays for the amplification of human genomic dna. Str identifiler plus pcr amplification kit user guide publication number 4440211 rev. Validation experiments to evaluate the performance of this ce consisted of a precision study, sensitivity study, genotype concordance, a two person mixture study, and signal normalization evaluation. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. Applied biosystems address, contact number of applied biosystems.

Licensing at applied biosystems, 850 lincoln centre drive, foster city, california 94404, or the licensing department, roche molecular systems, inc. Allele frequencies for nine str loci in africanamerican, chinese, vietnamese, and bangladesh populations. The protocol outlined in the quantifiler user s manual 19 was used for both automated and manual qpcr setup. Applied biosystems 1998 abi prismtm genescan analysis software 3. Str data for the 15 ampflstr identifiler loci in the moroccan population. Visit the web site to verify that you are using the most current version of this technical manual.

The applied biosystems 33xl gene tic analyzers getting started guide provides stepbystep instructions for pr eparing and analyzing a sample. Amplify the samples in a geneamp pcr system 9700 with the silver 96well block, a geneamp pcr system 9700 with the goldplated silver 96well block, a veriti 96well thermal cycler, or a proflex pcr system. Research day 2011 megan rommel comparative evaluation of. Nfstc dna training workshop literature amplification. The new kit includes the same primers and allelic ladder as the widely used identifiler ki. These improvements enhance the assay signaltonoise ratio and simplify the interpretation of results.

Applied biosystems quantstudio 6 flex realtime pcr system. An internal validation of the applied biosystems 8capillary 3500 genetic analyzer was performed using two commercial short tandem repeat str multiplex kits identifiler and identifiler plus. Applied biosystems 7900ht fast realtime pcr system and. Research day 2011 megan rommel comparative evaluation. Ampflstr identifilertm user s manual, applied biosystems, usa, 2001. These six amplification kits yielded the highest number of complete dna profiles for each substrate and body fluid with success rates greater than 60%. A comparison of ampflstr identifiler kit versus ampflstr. Powerplexr fusion system for use on the applied biosystems. Applied biosystems 37303730xl dna analyzers user guide vii preface about this guide audience this manual is written for principle investigators and laboratory staff who are planning to operate and maintain the applied biosystems 37303730xl dna analyzers. The address and contact number of applied biosystems is also used for applied biosystems real time pcr, applied biosystems taqman, applied biosystems products, applied biosystems manual, applied biosystems reverse transcription and applied biosystems thermal cycler. Provides information that may be of interest or help but is not critical to the use of the product. Genetic diversity of 15 autosomal short tandem repeats loci using. Ampflstr identifiler plus pcr amplification kit user guide pub. For all loci except amelthe same primer sequences as the ngm select kit and the identifiler plus kit including snpspecific primers for the vwa.

Str loci and multiplex kits becky hill research biologist, applied genetics group. Ampflstr identifilertm users manual, applied biosystems, usa, 2001. Ampflstr identifiler pcr amplification kit user guide pn. Applied biosystems 7900ht fast realtime pcr system and sds. Amplification kits globalfiler and globalfiler iqc pcr.

All samples were analyzed using genemapper id software version 3. The pcr amplification reaction mixture was prepared in a volume of 25 ll containing 10 llofampfstr identifiler plus master mix, 5 llof ampfstr identifiler plus primer set, and a maximum volume of 10 ll of target dna. The allele count per sample increases significantly due to the number of ministrs and robustness of the master mix included in the globalfiler kit, thus allowing for more meaningful database searches. Ampflstr profiler plus id pcr amplification kit page 5 of 32 safety documentation user attention words five user attention words appear in the text of all applied biosystems user documentation.

Development of a fast pcr protocol enabling rapid generation. Across injections, the data indicates that high standard deviation. Components designed to work fast allow the instrument to deliver results in as little as 30 minutes for samples on 96well microplates, the only format compatible with the instrument. Includes the user guide manual for the ampflstr identifiler plus pcr amplification kit. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems real fast. Ampflstr identifiler plus pcr amplification kit user guide. Ampflstr identifiler pcr amplification kit user guide 9 about this guide important. Pcr amplification kits, applied biosystems delivers its single most. Optimized for casework, the ampflstr identifiler plus pcr amplification kit enables forensic analysts to recover more interpretable results from a wider range of dna evidence samples with increased confidence. Applied biosystems quantifiler trio, yfiler plus and.

Applied biosystems user manuals download manualslib. Specifically designed with a user friendly, yet powerful interface, they are ideal for. Development of a fast pcr protocol enabling rapid generation of ampf. Ampflstr identifiler plus pcr amplification kit user s guide. This is the user guide for the ampflstr identifiler plus pcr amplification kit. Select the appropriate pcr cycle number ampflstr identifiler plus user guide 29. To view your gsa or va contract pricing, log in using your account number, or become a registered user by contacting one of our customer service teams.

Ccd camera user manual 212 mb abb king pro manual pdf download 51 mb a practical guide to witchcraft 8 mb. Within injections, the data shows no markers andor alleles with high standard deviation values exceeding 0. In addition, the identifiler plus master mix includes amplitaq gold dna polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup. Str identifiler allelic ladder, 9947a kit positive control dna and the 250basepair fragment from the internal size standard genescan liz500. Mar 06, 2012 development of a fast pcr protocol enabling rapid generation of ampf. Fitpro fit test software user manual hp vl420dt manual pdf file name size manual of exalted power sidereals pdf 99 mb. Str identifiler kit identifiler, applied biosytems, foster city. If you are viewing this page as a nonregistered user, the prices displayed is list. Ampflstr identifiler pcr amplification kit user guide. Applied biosystems 7500 realtime pcr system, desktop.

Comparative evaluation of manual extraction methods for the biologydna detail of the las vegas metropolitan police department forensic laboratory. If you are viewing this page as a nonregistered user, the prices displayed is list price. Applied biosystems pet and liz dyes expand the spectral detection range that can be used on abi prism genetic. Str minifiler pcr amplification kit was designed to amplify as.

Purpose the applied biosystems ampflstr identifiler pcr amplification kit user guide. Applied biosystems quantstudio 6 flex realtime pcr system, 96well fast. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. The identifiler kit uses a fivedye fluorescent system for automated dna fragment analysis. At this target, all peak height ratios are above 55% except one at the d19 locus. The 7500 fast realtime pcr system, manufactured by applied biosystems, is designed for fast performance and a variety of applications. Bowflex ultimate users manual downloadbowflex ultimate users manual. The stepone 48 well and steponeplus 96 well realtime pcr systems are remarkably simple to use. Str identifiler plus pcr amplification kit product bulletin publication number 112pb3002 co11504 0411. Travel guide 2007 maldives pdf 74 mb ccd camera user manual 212 mb. Pcr amplification kits, applied biosystems delivers its single most discriminating fluorescent strbased assay for human identification applications.

User friendly software includes plate setup wizards, multiplate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward. Each word implies a particular level of observation or action as described below. Oct 12, 2011 an internal validation of the applied biosystems 8capillary 3500 genetic analyzer was performed using two commercial short tandem repeat str multiplex kits identifiler and identifiler plus. Nist validation studies on the 3500 genetic analyzer. The 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. Applied biosystems offers a family of realtime pcr instruments with the 96well block format. Donna foskin from esr describes optimization for processing sexual assault kits using forensic dna analysis. By adding an additional dye, more loci can be multiplexed in a single pcr amplification as compared to the previous 4dye system. The improvements in the identifiler plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the identifiler kit. Applied biosystems fluorescent multicolor dye technology allows the analysis of multiple loci, including loci that have alleles with overlapping size ranges. Precision studies using the abi prism 3100 genetic analyzer have been conducted by performing multiple runs of the ampf. It is designed to help you quickly learn how to use the applied biosystems 33xl genetic analyzers. Applied biosystems identifiler plus user manual samsung l760 user manual machine user manual pdf.

Allelic ladder sizing precision was calculated from multiple injections of ladder run on the applied biosystems 3xl genetic analyzer. The recommended target dna input range for amplification using the identifiler plus kit is 0. The hid evolution system for automation of dna quantification. Since we pioneered realtime pcr over a decade ago, applied biosystems has continued to develop the technology to provide more powerful solutions to labs of all sizes. Profiler plus, cofiler, and sgm plus kits may quickly adopt the identifiler kit with complete confidence. As per the manufacturers instructions 12, dna amplification using the identifiler standard protocol is conducted in a 25. Each word implies a particular level of observation or action as. Intrarun and interrun precision data demonstrates the sizing reproducibility of the 3100 instrument for forensic applications.

Applied biosystems, abi prism, ampflstr, cofiler, genescan, geno typer. Str data for the 15 ampflstr identifiler loci in the moroccan. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The ampf lstr identifiler pcr amplification kit simultaneously amplifies 15 str loci plus the amelogenin genderdetermining marker in a single, robust pcr. The applied biosystems ampflstr identifiler pcr amplification kit user guide provides information about the applied biosystems instruments, chemistries, and software associated with the ampflstr identifiler pcr amplification kit. Dna casework technical procedures table of contents. The applied biosystems 7500 fast realtime pcr system represents the latest. Developmental validation of the ampfstr identifiler plus pcr. Ampflstr identifiler plus user guide 7 preface revision history purpose the applied biosystems ampflstr identifiler plus pcr amplification kit user guide provides information about the applied biosystems instruments, chemistries, and software associated with the ampflstr identifiler plus pcr amplification kit. Applied biosystems 2001 ampflstr identifiler pcr amplification kit user s manual.

Applied biosystems, 850 lincoln centre dr, foster city, ca 94404. Applied biosystems fluorescent multicolor dye technology allows the analysis of multiple loci, including loci that have alleles with overlapping. Prepfiler tm forensic dna extraction kit user guide. A comparison of ampflstr identifiler kit versus ampflstr identifiler plus kit in challenging bone samples by using normal and increased pcr cycle number. Pdf 1 avoid here maps i ve only used it 2 or 3 times in the last couple of weeks now you 8217 re just waiting patiently for people to come, hoping that all that hard work will pay off. Pdf, ebook, free books to download, user guard, ebook, books, manual, bowflex, ultimate, users, manual. Before using this product, read and understand the information the safety appendix in this document. Individual user logins can be added for up to four user. Applied biosystems 7500 manuals manuals and user guides for applied biosystems 7500. Pcr products were separated and detected on an abi 3500 genetic analyzer using a 36 cm array and pop4 polymer applied biosystems. Applied biosystems 2004a ampflstr identifiler pcr amplification kit. Development of a fast pcr protocol enabling rapid generation of. Allele frequencies for the 15 str loci included in the ampflstr identifiler.